Low testosterone can cause weight gain, lack of energy and a host health issues.
Before you can begin any form of hormone therapy—whether it’s Testosterone Replacement Therapy (TRT) or Human Growth Hormone (HGH) Therapy, you’ll first need a thorough evaluation of your hormone levels. At The HGH Therapy Doctor, we require blood testing as the first step in determining whether hormone deficiencies may be at the root of your symptoms.
Our specialized hormone clinics use a combination of targeted and comprehensive hormone panels to detect imbalances, with a specific focus on testosterone and HGH. These panels help our physicians understand the full picture of your hormonal health, including the function of the hypothalamic-pituitary axis, which governs the production of key hormones such as growth hormone and testosterone.
Before any blood work is ordered, every patient at The HGH Therapy Doctor undergoes a detailed physical examination and medical history review. You’ll be asked to describe your symptoms—how they started, how long you’ve had them, and how they may have changed over time.
While there are definitely symptoms of low testosterone such as weight gain, loss of energy, disturbed sleep, sexual health issues and cognitive difficulties – only a blood test can confirm the diagnosis.
Testosterone is essential for maintaining a man’s vitality and well-being, and levels naturally decrease as men age. Low testosterone (Low T) can negatively affect multiple aspects of life, including physical, emotional, and sexual health.
A proper diagnosis of low testosterone includes a physical exam, a review of your symptoms, and a blood test. Common symptoms include:

A testosterone blood test is performed to measure the amount of testosterone circulating in your bloodstream. Normal ranges are typically between 300 and 1,000 ng/dL, but your levels can fluctuate during the day, usually peaking in the early morning. That’s why we tend to schedule testosterone testing in the morning for the most accurate results.
Although some home testing kits rely on saliva samples, blood testing remains the gold standard. A comprehensive testosterone blood test provides more accurate information about your total testosterone and free testosterone levels and helps determine the best course of treatment.
While any medical provider can order this test, working with hormone specialists like those at The HGH Therapy Doctor ensures a deeper understanding of age-related hormone loss and more targeted treatment options.
For most testosterone tests, no special preparation is required, though timing is important. The majority of testosterone in your bloodstream is bound to proteins like SHBG (sex hormone-binding globulin), while a smaller portion exists as free testosterone. Both are considered bioavailable.
Most basic testosterone panels test for total testosterone, but to get a more precise view of your hormonal profile, your HGH Therapy Doctor provider may order a free testosterone test as well. This helps us better tailor your treatment plan to your individual needs.
Yes. Although testosterone is often labeled a “male hormone,” women also produce and rely on testosterone for healthy sexual function, energy levels, and mood. In women, testosterone levels tend to drop more significantly around menopause. For women experiencing symptoms like low libido, fatigue, or mental fog, a testosterone blood test may reveal an underlying hormonal imbalance.
Testosterone levels naturally fluctuate throughout the day, especially in men, so timing and context are key to proper diagnosis. At The HGH Therapy Doctor, we typically test testosterone levels in the early morning when they are at their peak.
Here are standard reference ranges for total testosterone:
In addition to total testosterone, your provider may also check free testosterone levels, which represent the portion of testosterone available for use by your body. Even if total testosterone appears normal, low free testosterone could still result in symptoms.
It’s also important to interpret these results based on symptoms, not just numbers. Some men feel symptomatic with levels around 350 ng/dL, while others may feel fine until they drop below 250 ng/dL. Likewise, women with testosterone at the low end of the range may experience fatigue, low libido, and mood instability.
At The HGH Therapy Doctor, we combine hormone testing with in-depth consultations to make sure we address the root cause of your symptoms—not just treat a number on a lab report.
Testing for your testosterone levels and other hormones and biomarkers related to testosterone and testosterone production is done with a simple blood draw. A healthcare professional will draw a blood sample, usually from a vein in your arm. The blood sample is then analyzed on-site or sent to a laboratory for analysis.
There is no special preparation needed for the blood test that doctors use to see if you have low testosterone. The typical testosterone blood test will look for your total testosterone. In some cases, to provide a more precise diagnosis of your body’s ability to produce testosterone, your doctor will also order a “free” testosterone blood test.
If the results of your testosterone test reveal that you have low testosterone, you will likely be prescribed a program of testosterone replacement therapy.
Low testosterone is a common problem in men over 40.
If the results of your testosterone testing indicate that you have low testosterone, depending on your presenting symptoms and medical history, your doctor may prescribe testosterone injections. While testosterone therapy can be given in other forms, our doctors have found that testosterone injections are the safest and most effective form of testosterone therapy.
Higher-than-normal testosterone levels are a much more uncommon issue than low testosterone. If your testosterone blood test results indicate that you have high testosterone levels, there is usually some kind of underlying cause, such as testicular cancer or some other type of tumor, genetic disorder, or syndrome. Further testing will be ordered to determine the root cause of the issue, and treatment will be prescribed accordingly.
Blood tests are crucial for diagnosing low testosterone. The following tests are commonly used:
A blood sample is taken to measure the total testosterone level in the body. Testosterone levels fluctuate throughout the day, so multiple samples may be required. Typically, a morning blood sample is preferred when testosterone levels are typically highest.
Along with total testosterone, the measurement of free testosterone (unbound and available for use by the body) can provide additional information about testosterone levels.
SHBG is a protein that binds to testosterone and regulates its availability in the body. This test measures the level of SHBG in the blood. Elevated SHBG levels can lead to lower levels of free testosterone.
Luteinizing Hormone (LH) and Follicle-Stimulating Hormone (FSH) are two important hormones involved in the regulation of reproductive function in both men and women. In men, these hormones play a crucial role in the production of testosterone and sperm. Testing LH and FSH levels can provide insights into hormonal balance and potential underlying issues, and are often run at the same time as a testosterone blood test.

Interpreting testosterone test results requires considering various factors, including age, symptoms, and other medical conditions. It’s important to note that testosterone levels can vary throughout the day and may decline with age. Reference ranges provided here are approximate and can vary depending on the laboratory:
If your testosterone levels fall below the reference ranges and you are experiencing symptoms, further evaluation by your doctor is necessary. Your healthcare provider may repeat the tests to confirm the results and also consider factors such as age, overall health, and the presence of other conditions.
It’s important to remember that testosterone levels can be influenced by various factors, including stress, illness, obesity, certain medications, and chronic conditions. Your healthcare provider will assess your specific situation and determine the appropriate course of action, which may involve additional tests or treatment options.
Always consult a healthcare professional to interpret your specific test results and guide you through the diagnostic process for low testosterone. They will provide personalized advice and recommendations based on your individual circumstances.
Particularly for men over 40, it is important to track changes in testosterone levels over time because the older you get, the more your testosterone level drops. The frequency of testosterone level testing for men over 40 can vary depending on individual circumstances and symptoms. However, a general guideline is to have a baseline testosterone level test around the age of 40 and then periodically monitor it every few years thereafter. If symptoms of low testosterone or other concerns arise, more frequent testing may be necessary.
It’s crucial to remember that the results of your testosterone blood tests alone may not provide a complete diagnosis of your testosterone levels or other hormone imbalances. Interpretation of your blood tests should be made in conjunction with other critical hormone levels, medical history, physical examination findings, and additional tests if necessary. Consulting with a healthcare professional, preferably one who specializes in age-related hormone issues like those at our clinics, is essential for proper evaluation and guidance based on individual circumstances.
It absolutely matters where you get your hormone levels tested. While at-home tests can offer convenience and potentially lower costs, they may not always provide the same level of accuracy or be interpreted in the context of your overall health by a qualified professionals like the caring specialists at The HGH Therapy Doctor. We know no two patients seeking TRT are the same, and neither are their needs or wellness goals. Using state-of-the-art diagnostics, we design a treatment plan tailored to your unique hormonal profile and distinct lifestyle.
When you choose The HGH Therapy Doctor near you for HRT, you’re not just receiving treatment, you’re gaining a partner committed to helping you thrive.
Discover the difference our personalized, compassionate care can make in your life today. Contact Us to see about getting your testosterone levels tested.
1. Why do I need a blood test before starting hormone therapy?
Hormone therapy isn’t something that should ever be started blindly. A blood test gives your provider a clear view of your hormone levels and helps determine whether a deficiency or imbalance is the root cause of your symptoms. At The HGH Therapy Doctor, your treatment plan will always be based on lab-confirmed results to ensure safety and effectiveness.
2. What’s the difference between total and free testosterone in a blood test?
Total testosterone measures all the testosterone in your blood, including what’s “bound” to proteins and what’s free. Free testosterone represents the unbound portion that your body can actually use. Both levels are important, and your The HGH Therapy Doctor provider may test for both to get a complete picture of your testosterone status.
3. When is the best time of day to take a testosterone blood test?
Testosterone levels are highest in the early morning—usually between 7:00 and 10:00 a.m. That’s why The HGH Therapy Doctor schedules testosterone blood draws in the morning to get the most accurate and consistent readings.
4. Can I eat or take medications before my hormone blood test?
It depends on the specific test. For most testosterone and IGF-1 tests, fasting is not required. However, some HGH stimulation tests may require fasting or medication restrictions. Your provider at The HGH Therapy Doctor will give you clear instructions ahead of your appointment based on the type of testing being performed.
5. How long does it take to get hormone blood test results?
Most hormone blood test results are available within a few business days. Once your results are in, a The HGH Therapy Doctor provider will review them with you, explain what they mean, and walk you through your personalized treatment options if needed.
6. Can women get hormone blood testing, too?
Absolutely. Hormone imbalances affect both men and women, especially with age. Women may experience declines in testosterone, estrogen, progesterone, or growth hormone—all of which can be evaluated through blood testing at The HGH Therapy Doctor. Our physicians are highly experienced in hormone therapy for both men and women.
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